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Test Compendium

2582: QUANTIFERON-TB GOLD

Test Code

  • 2582

Test Synonym

  • Quantiferon-TB Gold

Minimum Volume

  • 0.8 mL each

Specimen Requirements

  • Whole Blood

Container

  • Quantiferon Tb Sample Tube, TB

Storage

  • Ambient

Turn Around Time

  • 4 days

Special Instructions

  • This test is time-sensitive and specimens must be received in the laboratory within 70 hours after incubation by the client (no more than 94 hours from the time of collection). Specimen collection times will vary depending on logistics from the incubating

Additional Information

  • Refer to collection instructions included with draw kit. Special specimen collection kit contains three gel-barrier tubes: gray-top/white ring (nil), red-top/white ring (TB antigens), and purple-top/white ring (mitogen). All three tubes are required for a single test result. Each tube is designed to draw only 1 mL and fill time may be longer than other blood collection tubes. Because of the limited vacuum in these tubes, use a needle and holder (NOT a butterfly) to collect QuantiFERON(R) specimens. If a butterfly is required, first collect other required tubes or use another Vacutainer(R) tube to purge the butterfly line of air and then proceed with drawing the In QuantiFERON(R) tubes. Fill tubes to the black fill line on the tube. If tubes are underfilled or overfilled (see kit insert), immediately redraw a replacement tube. Following proper fill, label the tubes appropriately, and shake tubes 10 times just firmly enough to ensure the entire surface of the tube is coated with blood cells to solubilize the antigen on the tube walls. After shaking, the volume may fall below the fill line. Do NOT centrifuge or refrigerate specimens. Return each of the three properly filled, labeled and shaken tubes to the box labeled QFT(R) kit. Seal the top by removing tape from adhesive. Tubes should be incubated upright at 16-24 hours at 37 degrees C +/- 1 degree C in the box/kit within 16 hours of collection. Please indicate date and time of venipuncture on the test request form. If the specimen has been incubated for 16-24 hours at 37 degrees C +/- 1 degree C, please mark the top of the specimen box where indicated. INCUBATION: If blood is not incubated immediately after collection, remix the tubes by inverting 10 times immediately prior to incubation and within 16 hours of collection. Within 16 hours of collection, incubate the tubes upright at 37 degrees C +/- 1 degree C for 16 to 24 hours. The incubator does not require CO2 or humidification. Following incubation, ship the tubes to laboratory at 17 to 27 degrees C. Causes for rejection: specimen frozen; specimen more than 94 hrs old on receipt by the lab, unlabeled specimens not in the QuantiFERON kit box.

Specimen Stability

  • AMBIENT: up to 90 hours depending on the incubation REFRIGERATED: Do not Refrigerate - unstable FROZEN: Do Not Freeze - unstable