6166: PYRUVIC ACID, BLOOD
- Pyruvic Acid (Pyruvate), Blood
Turn Around Time
- 0.5 mL Clear supernatant from oxalated WHOLE BLOOD
- Draw blood into chilled gray-top tube. Immediately after blood is drawn, add 2 mL of whole blood from the gray-top tube to 2 mL of 8% perchloric acid (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). NOTE: Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained. Shake mixture vigorously for about 30 seconds. Refrigerate for five minutes to ensure complete protein precipitation. Centrifuge 5 to 10 minutes at approximately 1500xg. Immediately transfer the CLEAR SUPERNATANT to a transport tube. Label the transport tube as SUPERNATANT and send to the laboratory. NOTE: A second centrifugation of the supernatant may be necessary to obtain a clear protein- free solution.
- AMBIENT: 5 days REFRIGERATED: 7 days FROZEN: 7 days